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Samples were printed in quadruplicate onto nitrocellulose-coated glass slides (Path or SuperNova slides, Grace BInc.aBendInc., Bend, OR, USA), using an Aushon BioSystems 2470 Arrayer (Aushon BioSystems, Billerica, MA, USA) with 185 μm pins and a single touch.
Small amounts of each antibody solution were transferred to the surface of nitrocellulose-coated microscope slides (PATH slides, Gentel Biosurfaces) using a piezoelectric non-contact spotter (Biochip Arrayer, PerkinElmer Life Sciences).
A microarrayer (2470 Arrayer, Aushon Biosystems, Billerica, MA) was used to spot subnanoliter droplets of each antibody solution, prepared at 250 μg/mL in 1X PBS, on the surfaces of ultrathin nitrocellulose-coated glass microscope slides (PATH slides, Grace BendLabs, Bend, OR).
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Slide Path was one of his first runs at the resort.
The late-afternoon light, the larches, the sight of the rubbled slide path — the tranquility is deceptive.
BIGGEST CHALLENGE The slide path on the Saddle Trail required the use of handholds and extreme care.
Trails like the precipitous line Mr. Murphy skied — a funneling chute under a 40-foot cliff called Slide Path — are as difficult as the double-black-diamond runs at many Rocky Mountain resorts.
The slope angle of the sliding path was approximately 18° according to field investigations.
The basal friction coefficient was also calculated, combined with the slope angle of the sliding path.
The sliding path curvature generates: (a) an additional resistance to sliding, (b) a constraint force normal to the sliding trajectory.
The inertial forces are projected onto the sliding path, and the friction coefficients calculated, as shown in Fig. 6b.
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