The bones were cut using IsoMet® precision bone saw (Buehler Ltd., Lake Bluff, IL), and sections were mounted on plastic slides, ground and polished for histology and histomorphometry.
Spleens collected from KIKI or WT mice were mashed between 2 glass slides, ground with a 1-mL syringe pestle, then suspended in RPMI 1640 medium supplemented with 2 mM l-glutamine, 15% fetal calf serum and penicillin / streptomycin before filtering through a 75-µm nylon mesh.
These wafers are then mounted onto a microscope slide, ground and polished, and observed using reflected or transmitted light when the specimen is ground thin enough (<1 mm; Benedict et al., 2008).
The movement, combined with ample water, turned the sliding ground into a fast-flowing semiliquid.
Based on a shallow-buried coal seam covered with thick loose layers in hilly loess areas of western China, we developed a mechanical model for a mining slope with slope stability analysis, and studied the mechanism of formation and development of a sliding ground fissure by the circular sliding slice method.
Then the section was thaw mounted on a microscope glass slide (ground edges frosted, VWR international GmbH, Darmstadt, Germany).
The samples were mounted on slides and ground to a thickness of 80 μm.
The sections were mounted on plastic slides and ground to approximately 50 μm in thickness, and the surface was stained using a Giemsa stain modified for plastic sections [ 31].
She was also concerned about the plot, which early on had Jaymes on a downhill slide, losing ground to a young, blond crossover star played by Hayden Panettiere.
Polished balls of 52100 bearing steel were slid against ground discs in a self-mated Diesel fuel lubricated contact.
In the NCAA-ISP, ATs selected from a pre-set list of options including: player contact (e.g., collision between two players), surface contact (e.g., abrasion from sliding on ground), equipment contact (e.g. hit by stick), contact with out-of-bounds object (e.g., running into bleachers), non-contact (e.g., injury while running), overuse, illness, infection, and other/unknown.
