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Tissue blocks and cytology specimens were obtained and slides cut at 5 microns.
Slides cut from paraffin embedded blocks were also used for H&E stains and for immunohistochemical characterizations.
IHC and FISH staining were done on TMA slides cut from two replicas of TMA blocks for scoring to reduce the number of non-interpretable spots.
For this purpose, a cumulative focus score (cFS) was evaluated on three slides cut at 200-μm intervals from each of a series of 120 salivary biopsies.
Slides cut in parallel to the IHC-treated sections were stained by hematoxylin and eosin for better identification of the different tissue areas.
The bottom areas were glued to microscope slides, cut into 300 μm sections with a diamond band saw (Exakt, Norderstedt, Germany) and then ground to 80 μm.
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Tumor tissue was microdissected in comparison with a similarly stained and cover-slipped reference slide cut from each tissue block.
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