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After sealing both slides bottom and middle) with UV curable epoxy, stripper was used to remove the positive photoresist from the microchannels.
Z-stack images were collected from the contact site where T cells touch the cover slides (bottom) to the top of the cells where no signals were detected (top).
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In spite of the code's broad application, there are some failure modes such as slide bottom, elephant-foot buckling, sloshing and uplift needing more evaluation.
The tooling geometries consisted of a so-called "simple" design with no moving channel members and a "complex" design with a sliding bottom floor.
MOPS agar layers in cover slide bottom chambers were prepared following previously described methods [50].
Current plant cell methodologies place cultured cells mostly on flat, two-dimensional (2D) surfaces (microscope slide, bottom of a culture dish) where they cannot interact with 3D environments.
The residual baked photoresist was removed using stripper for 60 s leaving the three electrodes created on top of the glass slide bottom).
In brief, experiments were performed in 50 mM Tris buffer (pH 7.0) in a total volume of 20 μl in 384-well plates with a cover slide, bottom (Greiner Bio-One, Germany).
All experiments were performed in 50 mM Tris-buffer (pH 7.0) in a total volume of 20 μl in 384- or 96-well plates with a cover slide bottom (Evotec-Technologies, Germany).
Weighed down by debt and a flat economy, Dean struggled for several years as its stock price continued to slide, bottoming out near $7 in 2010.
To validate this improved "TIR" method, we introduced a half-glass/half-Ag patterned glass slide as the bottom slide (see the central panel in Figure 2a) to fabricate a blue-green rainbow grating at the incident angle, φ2, of 5°.
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