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On 1/9 of trials, the target slide was replaced by a blank slide (target-absent trial).
After spreading k562 cells (1 × 10/mL) onto glass slides as target antigen, the slides were incubated with fresh serum for 30 min followed by three 10 min PBS washes.
A 1951 USAF glass slide resolution target (Groups 0-3) was used to measure the limiting resolution of both the high-magnification probe and the wide-angle probe.
These came with slide shows targeting vulnerable Democrats, party talking points and discussions of doling out tax-paid "assets" of government.
Another slide suggests targeting WikiLeaks' supporters, including Salon.com journalist Glenn Greenwald.
Rabois explained that Slide mainly targets Fortune 50 companies and sells ad space almost exclusively on a CPM basis (no performance-based advertising or CPIs).
Prior to staining, heat-induced antigen retrieval was performed by placing the slides into target retrieval solution, high pH (DakoCytomation), and steaming them in a commercial vegetable steamer at full temperature for 30 minutes (CEBPD) or by pressure cooking in TRIS/Citrate (pH 6) for 8 minutes (alpha-smooth muscle actin, ACTA2).
Antigen retrieval was carried out by microwaving the slides in target retrieval solution pH 9 (DAKO).
Friedman et al. have used colocalization single-molecule fluorescent spectroscopy (CoSMos) to assess the contribution of 1D sliding in target finding using the σ RNAP holoenzyme.
Antigen retrieval was performed by heating the slides in Target Retrieval Solution, pH 9.0 (Dako Denmark, Glostrup, Denmark) at 97°C in a water quench for 5 min for the interstitial and cell surface antibodies or with boiling citrate buffer, pH 6.0 for 20 min in a microwave oven for nuclear markers.
In order to reuse the slides, hybridized targets were stripped off by using a modified version of the protocol used by Hahnke et al [15] as described in [13].
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Justyna Jupowicz-Kozak
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