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For each slide specimen, 100 pollen grains were chosen at random to assess their germination ability under a binocular microscope, and the count was used as the measure of fertility for each individual plant.
To calculate the forward SHG (FSHG) to BSHG ratio (FSHG/BSHG), both FSHG and BSHG were captured simultaneously above (BSHG) and below (FSHG) the slide specimen in two channels using the microscope setup previously described [ 30].
Thus whole slide specimen corresponding the core of Spiral Array was not available and we could not review and reclassified ADC into histological subtypes in new classification of lung adenocarcinoma proposed by International Association for the Study of Lung Cancer IASLCC).
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Two of the slide specimens bearing Darwin's name were gathered on the island of Chiloe in the Chilean archipelago.
Slide specimens were prepared by sectioning the tissue and staining with hematoxylin-eosin stain.
Finally, while findings on TMA specimens are promising, future work could compare the performance of Menacalc on TMAs to that seen on whole slide specimens as a step towards use in clinical practice.
While we believe that our MQIF method will be suitable for clinical usage to identify patients that are at high risk for developing metastasis, it awaits testing on routine whole slide specimens.
While the data were largely derived from the average of two or three spots, the assay has not yet been evaluated on whole slide specimens, as would occur in a true clinical setting.
The points were calculated and analyzed statistically for each slide of specimen.
The alignment of the LED was also checked using a standard fluorescent test slide (Convallaria specimen, Leica).
Two slides per specimen were prepared, and 25 cells per slide, i.e., 200 cells per exposed duration, were scored randomly and analyzed using an image analysis system (Komet 5.5 Kinetic Imaging Ltd., Nottingham, UK) attached to a florescent microscope (Leica) equipped with appropriate filters.
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