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The primary astrocytes were cultured on 10 mm circular slide slides.
This minimized the variations among biological and technical replications, including animals, fluorescence dyes, sample combinations, blocks on a slide, slides, and experimental conditions (Table 1).
After the final washing step and spin-drying of the slide, slides were scanned using an Axon scanner at maximum laser power (Axon Instruments, Union City, CA) at both 532 and 635 nm.
Flies were left to recover in a petri dish for 10 15 min before being tapped onto a soot-covered glass microscope slide (slides were held over a candle flame until covered in an even coating of soot).
Immediately before placing explants on the slide, slides were washed with HBSS and DRG culture medium (Neurobasal medium [21103-049, Invitrogen], 1× B27 [17504-044, Invitrogen], 100 U/ml penicillin/streptomycin [15140-122, Invitrogen], 2 mM L-glutamine [25030-081, Invitrogen], and 35 mM glucose).
The NHLS provides the spatula, glass slides, slide fixative, and laboratory request form to the clinics.
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INSTRUCTOR: The routine wasn't even difficult: five, six, seven, eight, kick-ball-change, half step, full step, kick-ball-change, side-lock, pull the arms, grand jeté, kick-ball-change without the ball, tendu, break, underhop, slide, slide, slide, slide, slide, slide, slide.
"SLIDE SLIDE" and then "SLIDE" some more into corners, goalies, players, and the puck.
If you use the 11 card and you change place, but somehow landed on a slide, slide to the end!
It was seen in slides; slides were popular at the N.Y.U.
These segments are categorized into: baseline (Base), first 5 min of viewing slides (Slides 1), second 5 min of viewing slides (Slides 2), mental stressor (Stress), and in recovery (Recv).
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