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His photographic skills were instrumental in preparing slide samples.
Ozanian also likes that Cytyc has been using its cash to develop new products, like its ThinPrep Imaging System, a computer-assisted device that screens slide samples prepared by the ThinPrep test for the presence of cancer cells.
After washing (2× in PBS) and air drying, slide samples were incubated with rabbit anti-human Immunoglobin FITC (F0200, DAKO, Denmark) in 0.05% w.v Evans Blue (3169, Merck, PBSS for 30 minutes at room temperature.
36 corresponded to slide samples and 77 to punch samples.
Finally, 7.5 ml blood was used to create two glass slide samples for microscopic analysis.
In negative cases, two slide samples were additionally stained with an anti-pancytokeratin antibody (AE1/AE3, Dako, Carpenteria, CA, USA; 1 200).
Similar(45)
After sliding, samples and reagents of various concentrations could be mixed for multiplexed reactions.
In addition, smear slides samples provide only semiquantitative information.
After mounting onto Superfrost Plus glass slides, samples were de-paraffinized and rehydrated as described [ 31].
After dissected discs were mounted on slides, samples were given an arbitrary label and all subsequent imaging and data analysis was carried out blind.
To ensure DNA spotting was uniform across the slides, samples were taken from each batch of microarrays and probed with Panomer™ 9 random oligodeoxynucleotide conjugated with Alexa Fluor® 532 (Molecular Probes, Invitrogen).
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