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Histopathological slide preparations of liver were made by routine microtechnique method of tissue sectioning and staining with haematoxylin- eosin for histological examination.
The harvest and slide preparations were completed according to standard methods and G-banded metaphases were analyzed.
These were identified to species using microscope slide preparations of sclerite morphology, using established octocoral protocols [13].
Slide preparations were made by dropping the fixed cell pellet onto cold, wet slides and air-dried.
The authors wish to thank Jenny Groeltz of the Histopathology Laboratory College of Veterinary Medicinee, ISU) for histopathology slide preparations, and Ting Li and Justin Breitbach for technical assistance.
Nick-translated, spectrum red-labeled tumor DNA and spectrum green-labeled normal DNA were coprecipitated with excess unlabeled human Cot-1 DNA (GibcoBRL), denatured, and hybridized to the normal metaphase slide preparations.
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ALF prepared samples for histological slide preparation, collected morphometric of histological slides and conducted statistical analyses of the data.
Slide preparation is done according to a modified method of Tice et al. (2000).
H. sp. was identified based on morphology, using images taken from a slide preparation outlined below.
Furthermore, during slide preparation, factors such as fixative evaporation rate and preheating of slides affect the quality and quantity of mitotic chromosomal spread (Moore and Best 2001).
Identification of H. sp. was subsequently based on images from a slide preparation (described in methodology), which were subsequently assessed by Dr. S. Spaulding (Colorado State University) for identification.
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