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In this study, to ensure the accuracy of epithelium percentage estimated, adjacent H&E slide of approximately 15 sections of prostate tissues (6 μm each) was used for epithelium estimation.
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The upper parts of the blocks were removed using a diamond band saw (Exakt, Germany), leaving slides of approximately 300 μm of MMA including the specimens.
The detected flourescence was adjusted by altering the photo multiplying tube (PMT), this ranged from 700 820 (arbitrary units) to give an intensity ratio of the slides of approximately one.
Each microarray slide consisted of approximately 13,000 elements including target genes and quality controls.
The results of our study are comparable to human studies reporting a rate of manual slide review of approximately 25% [ 11, 12].
The mixing of 30 wt% TiO2 NPs into the PBA matrix generated a superhydrophobic surface (static water contact angle: >150° with a sliding angle of approximately 1°).
The use of coatings with an Al2O3 intermediate layer at the lower sliding speed of approximately 0.5 m/s deteriorates the wear resistance of the coated specimens, but its protective role becomes more important at the high speed conditions in comparison to an uncoated carbide specimen.
Slides were recoded, and another 5,000 sperm were scored from the bottom half of the slide, for a total of approximately 10,000 sperm per slide.
Previous years have shown a slide positivity rate (SPR) of approximately 30% in the high-transmission season [ 20].
The rock avalanche initiated as a planar sliding failure with volume of approximately 91 Mm3.
Normal horse serum (DAKO) was used to block slides for approximately 20 min prior to the application of antibody.
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