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The females of T. cinnabarinus were selected for bioassay using a slide dip method.
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In Figure 1, tissue slides from a colorectal adenoma were preserved by slide dipping followed by immunohistochemistry for MSH6 at 2 months, 4 months and 7 months.
The cell suspension was spread on a slide dipped in the fixing solution (2% paraformaldehyde, 0.05% Triton X-100) and the slides were dried for 1 h in the dark.
Oocytes and spermatocytes were dispersed on a glass slide dipped in 1% paraformaldehyde fixative with 0.1% Triton X100.
To prepare chromosome spreads, purified PGCs and oocytes were put on a glass slide dipped in the fixation solution of 1% PFA in DW (pH 9.2) containing 0.15% Triton X-100 and 3 mM dithiothreitol (Sigma-Aldrich).
The mites were tested annually for high sensitivity to organophosphate insecticides, using the glass slide-dipping method according to FAO standards.
The mites were treated with β-sitosterol using the glass slide-dipping method according to the FAO [ 19]. β-sitosterol (Sigma-Aldrich, St . Louis MO, USA) was diluted in sterile distilled water containing 1% Tween 80 (Sigma-Aldrich) and 0.6% DMSO (Sigma-Aldrich).
One-inch tubules were then placed in 20 μl of sucrose solution (100 mM sucrose, adjusted with NaOH to pH 8.2) and mixed to a cloudy suspension, which was then spread onto two slides dipped in a freshly prepared formaldehyde solution (1% formaldehyde, 0.15% Triton X-100 in water adjusted with sodium borate to pH 9.2).
Act like a crab (sorta) Now jump and slide, now dip, that's right!
Then a chemically treated glass slide is dipped into the liquid and slowly drawn out.
Related slide show: Dipping in Vermont's natural pools.
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