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In order to automate the brain tumor (Glioblastoma) detection, we proposed a novel approach of skull stripping for axial slices derived from MRI.
For this, morphine or salicylate was applied in vitro to the hippocampal slices derived from chronically drug-treated or saline-injected rats and drug-induced changes in evoked field potentials of area CA1 were evaluated.
In ICA, the third dimension K of the three-way array corresponds to the number of covariance matrices at different lags, or the number of matrix slices derived from a cumulant array.
For patch clamp recordings standard procedures were also used to prepare 300 µM thick horizontal slices derived from juvenile mice (P18 28) (mEPSCs, mIPSCs) or coronal slices derived from adult mice for LTP experiments.
Most studies have examined the effects of estrogens on primary neurons or brain slices derived from animals.
Electrophysiological experiments were performed on hippocampal slices derived from adult mice that received a tetanus to produce LTP at the Shaffer collateral-CA1 connection.
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While numerous peptides from both GroEL1 and AccD5 could be identified from gel slices deriving from the mc155 strain, only AccD5 peptides could be detected in the sample obtained from the groEL1ΔC strain (Additional file 1).
Counts of nuclei per field in untreated compared with treated slices deriving from the same tumour allowed estimation of the anti-neoplastic activity of individual drugs on an individual tumour.
Several 2 mm thick transverse slices were taken and processed as follows: an area of lung slice derived from the most caudal region of each treated segment was fixed in 10%% neutral-buffered formalin and processed in paraffin for histopathology assessment.
Cross-sectional cord area was measured on a series of five contiguous axially reformatted slices (3-mm slice thickness) derived from the T1-weighted volume using a well-established semi-automated segmentation method (Losseff et al., 1996; Freund et al., 2010).
Making use of organotypic slice cultures derived from the mouse neocortex, drug effects were assessed by extracellular voltage recordings of network activity at basal cholinergic tone and during simulated cholinergic crisis (high cholinergic tone).
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