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Slices containing the dentate gyrus of the hippocampus were stained for doublecortin (DCX).
The brains were next rinsed with PBS and 100 µM coronal slices containing VTA were collected.
However, some testers clearly preferred crispy and darker bacon slices containing higher HAA concentrations.
To examine this hypothesis, brain slices containing the locus coeruleus (LC) from animals expressing the FLAG-D2 were imaged.
Recordings directly from neurons involved in behavioral state control are made using the whole-cell patch-clamp technique in brain slices containing the region of interest.
Brain slices containing the SN from animals expressing FLAG-tagged D2 receptors were incubated in the anti-FLAG M1 antibody conjugated with AF594 for 30 minutes.
Coronal slices containing the dorsal striatum were allowed to recover for at least 1��h at room temperature in carboxygenated aCSF.
Coronal brain slices containing medial prefrontal cortex (mPFC) or primary somatosensory cortex (SCx) were prepared from adult mice administered WIN or vehicle during adolescence (Figure 1a).
Conventional 2-dimensional (2D) radiotherapy planning is performed with sets of beams oriented with respect to axial computed tomography (CT) slices containing relevant anatomical information.
The brains were then quickly removed and 300 μm thick slices containing the NAc were cut either in the horizontal or sagittal plane using a vibratome, while submerged in the physiological saline solution.
250 µm horizontal brain slices containing VTA were incubated for 30 min at 35 °C in a chamber filled with artificial cerebrospinal fluid (aCSF) and stored at room temperature for the remainder of the day.
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CEO of Professional Science Editing for Scientists @ prosciediting.com