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All gliomas showed variable numbers of invasive tumour cells within the slice tissue.
A similar objective (CFI Apo 60x Oil λS, Nikon Japan) with enhanced transmission was used for imaging the brain slice tissue.
After 14 days, some GFP-positive OPCs were attached only to the culture membrane, or the edge of the slice, but others penetrated into the slice tissue and myelinated axons (Fig. 2F).
Shown are the slice mass (absolute slice tissue mass, in grams), and the mass in hyperinflated compartments (Hyperinflated), in normally aerated compartments (Normally), in poorly aerated compartments (Poorly) and in the non-aerated compartments (Non-aerated).
The increase in the slice tissue mass as PEEP decreased, as well as from expiration to inspiration (Table 2), may reflect cephalo-caudal shrinking of the lungs or may result from the fact that, at high levels of PEEP, the VT may distribute outside the field of view of the CT scanner.
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Last month, I had a gum graft, where my periodontist sliced tissue from the roof of my mouth and relocated it to the frail gums beneath my lower front teeth.
The protocol that finally worked involved simply slicing tissues into pieces with scalpels, then immersing them in trypsin overnight.
In his spare time, Purkinje improved techniques for preparing tissue samples, using a microtome--an instrument that slices tissues into very thin samples.
Sagittally sliced tissues were labeled with DiI at E12.0 and incubated for 36 h.
Global microarray expression analysis of in vivo and in vitro (ovary slices) tissues exposed to FAD revealed significantly different responses.
Enhanced green fluorescence protein expression in the blood and sliced tissues was examined under a confocal laser-scanning microscope (LSM 510, Carl Zeiss, Thornwood, NY, USA).
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