Sentence examples similar to slice restriction from inspiring English sources

In-slice restriction digests were performed on gel slices containing individual plasmid bands excised with a razor blade.

The DNA in agarose plugs was restricted with 40 U/plug-slice of XbaI restriction enzyme (Roche, Indianapolis, IN), following the manufacturer's recommendations.

To compare the reaction efficiency of SLiCE with that of restriction enzyme-digested vectors, the pET23a and pET23d vectors were linearized by digestion with restriction enzyme and purified using a Gel/PCR Extraction Kit (FastGene) following agarose gel electrophoresis.

After washing, the plugs were sliced and then digested in fresh restriction buffer with the enzyme SmaI (50 U/ µL) at 25°C for 12 h.

Coding efficiency is reduced because of the restriction of intra prediction across slice boundaries.

Each slice was pre-incubated with 250 μl of restriction buffer recommended for the enzyme for 30 min at 4°C and then replaced with 250 μl of fresh buffer containing 20 units of restriction enzyme.

Among those methods: removing the restriction enzymes that M. capricolum uses to slice up foreign genetic material (which led to the recent failure) or attaching methyl groups to the synthetic genome to protect it in the cell.

DNA restriction fragments were purified from agarose gel slices using the ZymoClean kit (Zymo Research).

A slice of each plug was cut and incubated in 400 µl restriction buffer containing 50 U XbaI (Takara Bio) at 37 °C for 2 h.

The standard SLiCE reaction solution comprised the following components: 10 ng linear vector (PCR amplified or restriction enzyme-digested), an appropriate amount of insert DNA (1 1 to 3 1 molar ratio of insert to vector), 1 μL 10× SLiCE buffer, 1 μL SLiCE extract, and sterilized distilled water to a total volume of 10 μL.

Slices of plug (1X5 mm) were digested by using 30 U of the restriction endonuclease enzyme Spe I (GIBCO BRL, Burlington, Ontario, Canada) for 1.5 hours.