However, the limited number of microspheres present in a single slice does not allow a reliable determination of perfusion values for different slice regions.
Synthetic image series in a Siemens star pattern with 50 non-empty slice regions were generated such that the maximum width of each slice (on the outer diameter) equals 213 nm.
In all three slice regions, kainate significantly increased the occurrence of pyknotic nuclei (observed with 4′,6-diamidino-2-phenylindole (DAPI) staining), a process already detectable at the end of 1 h application of kainate and intensified 24 h later despite intensive washout.
(a) Original slice, (b) binary mask computed by thresholding with μ k), (c) object of highest area (column #70 highlighted), (d) one-dimensional profile corresponding to the column #70, (e) outcome of the proposed algorithm, (f) object of highest area, and (g) masked slice (region of interest).
In the case of the whole slice region (C = 88, α = −88 and λ = 0.3131), the percentage of activity excreted through the renal pathway is 88%, and for the threshold VOI (C = 266, α = −266 and λ = 0.0598), the fitted parameters are not physiologically meaningful (i.e. renal excretion of 266%).
For the distal femur, a 30 mm (30 slice) region of interest (ROI) will be analysed starting 5 mm from the most distal point of the femur.
After reorienting the factor images along the heart axis to form vertical long-axis, horizontal long-axis, and short-axis-slices, regions of interest were drawn within the left ventricular cavity and myocardium on consecutive image planes and projected onto the dynamic 15O-water images to generate blood time-activity-curves.
Moreover, within tumor-implanted brain slices, regions far away from the tumor provided reliable controls for distinguishing tumor-induced effects from technical impacts.
They show changes in electrical impedance derived from a three-dimensional chest slice because regions out of the electrode plane also affect the measured electrical potential differences and influence the generated images [50].
The next day, brains were cut into 1 mm slices, mapped against a diagram of the whole slice, and regions to be studied were removed from the slices and fixed overnight in 2.5% glutaraldehyde in 0.1 M PB (Electron Microscopy Sciences, Inc., Hatfield, PA) at 4°C.
The end result of all CAD systems was a colour overlay on each MRI slice indicating regions of significant enhancement and providing details about enhancement type and extent.
