Sentence examples for slice reaction from inspiring English sources

aUnpurified insert DNA was directly used for the SLiCE reaction.

An equivalent volume to 1/20 of the PCR solution was used for the SLiCE reaction.

The SLiCE reaction mixture was incubated at 37 °C for the indicated time (5 120 min).

aOne microliter of unpurified insert DNA fragments in 20 μL PCR solution was directly used for the SLiCE reaction.

After the SLiCE reaction solution had been prepared, the SLiCE extract was thawed on ice and mixed gently.

One microliter of the SLiCE reaction solution was chemically transformed into 20 μL ECOS X Competent E. coli DH5α (Nippon gene, Tokyo, Japan) according to the instruction manual.

Various parameters were used to measure the efficiency of the SLiCE reactions under each experimental condition.

For in vitro slicing reactions, beads were incubated for 45 min at 37°C with 1 μM target RNA (5′-Cy3-ACCGUCUAACAACAGACUUUAAUGUAAUUGUGGAA-3′) in RLC buffer (20 mM Tris HCl 7.4, 50 mM NaCl, 3 mM MgCl2, 2 mM DTT, 0.5 mg/ml tRNA, 2 U/μl RNAsin (Promega)).

The SLiCE (JM109) reaction was performed for 10 min at 37 °C.

The SLiCE (JM109) reaction was performed for 60 min at 37 °C with 1 1 and 3 1 molar ratios of insert to vector for Prx IIE and G6PDH1, respectively.

The SLiCE (JM109) reaction was performed for 10 min at 37 °C with 1 1 and 3 1 molar ratios of insert to vector for Prx IIE and G6PDH1, respectively PCR-amplified insert DNAs have often been cloned into vectors digested at restriction enzyme cleavage sites as a standard protocol [ 32– 32].