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Similarly, the distinction between L5A and L5B could not be justified based on the cell density profiles (Fig. 3 A ). Yet, the VPM projection pattern, the anti-GAD67 immunofluorescence, and even the native slice observed in brightfield illumination clearly show a sharp boundary within L5 (Fig. 1 A, B ).
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Micrograph comparison of particle-free agarose and PVA slices, observed by scanning electron microscopy (SEM) at low magnification (Figure 1), has shown that both types of the cryogel monoliths had quite similar structures with evenly distributed interconnected pores, with an average pore size within the range 4 to 100 μm.
Figure 2 shows exemplary images of 20-μm-thick polypropylene slices observed with the polarized light microscope.
(A, C, E) CT slice images observed from the side, with sagittal sections of the IC at the middle.
The graphite exfoliated by microwaver was dispersed under ultrasonication for 1 h (Figure 3) and dropped in a silicon slice and observed by SEM, as shown in the thin flakes (Figure 3a,b).
However, circadian patterns of activity throughout the life of the slice were observed during the entire recording period (Fig. 1E).
The developed slice was observed under a light microscope.
After at least 24 hr, the epidermis was removed from the onion slice and observed using a Confocal Laser Scanning Microscope.
The 10 ms inter-stimulus interval produced a 8.1±6.3% facilitation in WT slices, but in KO slices we observed a 12.9±4.7% depression (Figure 1C; Burst 1, EPSP 2).
Slices were observed under inverted microscope (Leica DMI6000B inverted microscope, RBT).
Histological analysis showed that bacilli infecting lung tissue slices were observed in the alveolar septa, alveolar light spaces, near to type II pneumocytes, and inside macrophages.
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