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Vu,brain is best measured in vitro using the high-throughput brain slice methodology [11].
Data was identified in literature for Kd and Bmax, whereas a brain slice methodology was used to determine values for Vu,brain.
Borrowing in part from the "thin slice" methodology, we assessed outcomes in a 2 (deliberating vs. non-deliberating jurors) × 3 (length of videotaped testimony) between-subjects design.
In this study, a novel rat lung slice methodology has been developed and applied to study drug uptake in lung tissue, and the mechanisms by which this occurs.
Moreover, studies have investigated mechanistic pharmacokinetic/pharmacodynamic links using brain slice methodology [ 13, 16].
In view of this, Equation 3 can be reorganized to obtain Vu,brain corrected for the remaining aECF volume on the brain slice: (4) V u, brain = A brain − V i · C buffer C buffer · 1 − V i Once the brain slice methodology is established in the laboratory (Table 5), it can be used in a high-throughput manner.
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Slicing methodology itself has no obvious flaws, but its accuracy is dependent upon the three dimensional digital sculpture sliced by the algorithms.
This finding is consistent with recent work using cell culture and brain slice-based methodologies, which showed that miR132 increased spinogenesis [13].
To directly compare the size and complexity of dendritic arborization across species, we reconstructed pyramidal neurons from superficial L2 and L3 of adult mouse temporal cortex using the exact same methodology for slice preparation, dye loading, histological processing, and neuronal reconstruction as for the human neurons.
Figure 2 Schematic representation of the proposed methodology for slicing the observation block into M sub-blocks.
Optimization of pretreatments – blanching and additives was carried out for frozen pineapple slices using response surface methodology (RS M.
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