The average ± SD thickness of all the slices (excluding first and last slice) from five specimens was 5.28 ± 2.42 mm.
In the 3D videos, we also randomly selected at least one of its 2D z-slices, excluding empty slices.
In the same selected image, ROIs of the renal tumour and normal renal cortex were drawn manually (maximum 1 cm), lying within the structure of interest in each slice and excluding necrosis, calcifications, or cystic or any hemorrhagic areas.
The axial end slices (first and last slice) were excluded from the analysis.
The slice package excluded the dorsal frontal, parietal, and occipital cortices.
The 3 axial end slices (the first and last 3 slices) were excluded from the analysis.
In previous studies it is not defined either how the basal slices were selected, or if the basal slices were excluded or defined separately for end-diastole and end-systole thereby not including the long-axis motion.
Eight slices of exact equal size (excluding serum albumin protein band) from healthy and diseased sample sets were excised and subjected to trypsin digestion as described earlier [ 11].
For all participants, the most superior and inferior slices were excluded from the analysis, as a precaution against those slices shifting into previously unexcited regions.
