In this wide spectrum of environments, the slicing components may be provided by different vendors.
Accordingly, AGO2 and AGO3 of rice and Arabidopsis that possess DDD could still function as slicer components of silencing effector complexes.
To interrogate which of the OsAGO possessed the conserved catalytic residues and could potentially act as the slicer component of silencing effector complexes, we aligned the PIWI domains of all the OsAGOs using CLUSTALX.
To interrogate which of the predicted MtAGOs included the conserved catalytic residues and could potentially act as the slicer component of the silencing effectors complexes, we aligned the PIWI domains of all the predicted MtAGOs and AtAGOs using T-Coffee (Additional file 7).
As anatomical regions can never be restricted to an arbitrary chosen single MRI slice, these components clearly point towards analysis induced artefacts.
FDG-PET/CT imaging was performed using a dedicated PET/CT device with an enhanced axial bed coverage of 216 mm (TrueV®) and a 64-slice CT component (Biograph mCT 64®, Siemens Healthcare, Erlangen, Germany).
A PET/CT scanner with a 64-slice CT component (Gemini TF, Philips, Best, The Netherlands) was used.
Whole-body scans were performed on a combined PET/CT system (Discovery ST, with a 16-slice CT component; GE Healthcare Bio-Sciences CorPiscatawayway, NJ, USA).
As a starting point for analyzing how solutions might be contaminated with Zn through contact with various labware items I used the example of an experiment attempting to image Zn release from hippocampal slices, testing all components that came into contact with the physiological saline during the experiment [ 15, 17].
In addition to usual management and orchestration areas served by Virtual Infrastructure Manager, Virtual Network Function Manager and Network Functions Virtualization (NFV) Orchestrator, the authors have introduced a new element which is Inter-Slice Orchestrator and the highest orchestration element (NFVO) divided into two components: the slice orchestrator and the Service Orchestrator.
