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To determine whether RNA silencing can be initiated in agro-infected rice leaves by our method, we selected two endogenous gene targets namely Phytoene Desaturase (OsPDS) and SLENDER 1 (orSLR1 OsGAIGaI, a rice ortholog of the height-regulating gene GAI/RGA/RHT/D8) because of their well-documented loss-of function phenotype.
Using this protocol to transiently inhibit gene expression in leaf cells, we introduced hairpin RNA (hpRNA) T-DNA constructs containing gene specific tags of the phytoene desaturase (OsPDS) and of the SLENDER 1 (OsSLR1) genes previously proven to trigger RNAi of target genes in stable transformants.
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