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Analysis of sera from 1961 sheep, collected just before slaughtering from 62 farms in Sicily revealed a seroprevalence of 49.9% using a commercially available ELISA for Toxoplasma-specific IgG.
Tissue samples from a specific anterior limb muscle (Biceps brachii) were collected, immediately after slaughtering, from 25 beef cattle randomly selected in 10 different batches and stored in 2 mL RNAlater solution (Ambion, Monza, Italy) at −20°C until extraction.
Urine samples were collected during slaughtering from the bladder and each sample, without any preservative, was divided in 15 mL aliquots and stored at −20°C until the analysis.
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And after watching his family being slaughtered from afar, revenge was on his mind.
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A total number of 841 pigs slaughtered from March to September 2011 were investigated.
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During 1997 2007, blood samples from pigs that originated from breeding herds and that were slaughtered from the test stations were tested for H1N1 antibodies [ 12].
The sampling interval was obtained by dividing the total number of animals slaughtered from specific geographical origin within specific day by the estimated daily sample size [ 20].
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