Exact(6)
At slaughter samples of subcutaneous fat from the brisket (BF) and skirt muscle (pars costalis diaphragmatis; PCD) were procured for determination of chemical fat content, fat dissected from the muscle and for enumeration of adipocytes, less than 35 μm in diameter and to determine the average cell size in the dissected fat and from the BF by flow-cytometry of adipocytes fixed in osmium tetroxide.
At slaughter, samples of subcutaneous adipose tissue were collected, from which RNA was extracted.
At slaughter, samples of muscle from the gluteus medius were collected, snap frozen in liquid nitrogen and stored at -80°C until analysed.
In contrast, the meat samples of the males from farm D were all positive for Campylobacter, while only 75%to83%3% the slaughter samples were positive.
9 At slaughter, samples of s.c. adipose tissue (head tail) were obtained immediately after exsanguination, frozen in liquid nitrogen and then stored at −60 ºC.
Immediately after slaughter, samples of longissimus lumborum muscle and SAT for gene expression analysis were collected from the right side of carcass at the 5th lumbar vertebra level, rinsed with sterile RNAse-free cold water solution, cut into small pieces (thickness of ~0.3 cm), stabilised in RNA Later solution (Qiagen, Hilden, Germany) and subsequently stored at −80°C.
Similar(54)
Briefly biopsy samples of LM were collected from nine development stages (pre and postnatal) and a post slaughter sample.
This included a randomized pre-slaughter sampling and testing program conducted at high expense in Egypt (Rushton and Rushton 2009; Yrjö-Koskinen et al. 2010).
Freezing of products from all flock lots found positive on pre-slaughter sampling, and the price penalty to the producer for positive flock lots, ensured continued producer motivation to maintain high standards.
Following slaughter, blood samples were collected from each animal.
At slaughter digesta samples were collected from the stomach, ileum, caecum, proximal and distal colon.
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