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The excellent performance of the sensor comes from simultaneously fabricated layer by layer structure "target DNA + Hemin / Au-Luminol NPs / DNA* / sl DNA / TGA / QDs / MWNTs / GCE" mode which was based on the enhancing effect of luminol by G-quadruplex / hemin and Au nanoparticles and the quenching effect of CdSe/ZnS by G-quadruplex / hemin.
First, in neuroborreliosis, perivascular and vascular lymphocytic inflammation is associated with the presence of B. burgdorferi sl DNA [ 46].
As suggested for other gymnosperms, the large amount of SL DNA in Taxodium is likely the result of divergence among ancient repeat copies and gene/pseudogene duplication.
DNA analysis using nested PCR on ten seropositive and 16 seronegative aortic wall specimens did not detect any B. burgdorferi sl DNA.
The failure to detect B. burgdorferi sl DNA was unlikely to be due to technical problems, but a true negative finding, since PCRs in which the samples were spiked with B. burgdorferi DNA, yielded a product.
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Thus, we suggest a second reason for the enhanced binding affinity of SL-DNA conjugate, relative to SL for BoNT/B, resulting from an ionic interaction between DNA and BoNT/B (Supporting Information Figure S4).
Compared to monovalent SL-DNA conjugates, multivalent interaction of SL improved the affinity for BoNT/B, avoiding false positive signals and increasing the detection sensitivity to 600 aM BoNT/B in honey.
The SL binding site of recovered BoNT allowed SL-DNA conjugates to form BoNT-SL-DNA complex.
Among 19 drowned cases within 3 days postmortem, SL-DNA was detected in all of the blood samples from hearts with SL1 and AH-DNA was detected in several samples with AH1.
In a case of drowning in a bathtub, use of the conventional acid digestion method for diatom analyses and the PCR method for identifying plankton DNA revealed no plankton, but our PCR method for detecting bacterial DNA showed a positive result for SL-DNA in a blood sample from the heart.
Second, the SL-DNA conjugate is bound to the BoNT immobilized on beads in the form of a BoNT-SL-DNA complex.
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