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This would ensure a perfect match with the skull surface.
The stimulating electrodes were attached to the skull surface and the same stimulation protocol was applied.
The skull surface was exposed and holes drilled at the injection sites.
The following coordinates relative to bregma were used for injections: lateral ventricle, anteroposterior −0.1 mm, mediolateral −0.9 mm, dorsoventral from the skull surface −2.3 mm; PVN, anteroposterior −0.7 mm, mediolateral ±0.25 mm, dorsoventral from the skull surface −4.9 mm.
Gerbils were anesthetized using an isoflurane/oxygen mixture and an incision was made to expose the skull surface.
A spherically uniform sampling on the smoothed skull surface is performed afterwards.
Since the segmented skull surface is not sufficiently smooth, which will introduce errors in the subsequent shape description, we devise a surface smoothing method to improve the quality of the skull surface.
Precise analysis of the preferential alignment along the skull surface showed an elliptical distribution of the c-axis of biological Ap elongating along the suture inside the skull surface of both lamina exterior and interior.
Left: Distribution over skull surface of absolute gamma EEG magnitude, theta-cordance and alpha slow-wave index at 1 h of perfusion.
Four or seven pairs of Ag/AgCl stimulation electrodes were fixed to the skull surface bilaterally by conductive electrode gel (Fig. 4b, c).
Immediately prior to imaging, rats were anesthetized under 2% isoflurane, the skull surface was exposed and the landmark suture Bregma located.
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