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Open image in new window Fig. 5 Molding of the skull post vaginal delivery Immediate post-delivery appearances of the skull on head CT.
An X-ray of my skull, post chunk-loss.
Brains were removed from the skull, post fixed for 12 h, and cryo-protected for 24 h in 30% sucrose dissolved in 0.1 m phosphate buffer (PB; pH 7.4).
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Brains were dissected from the skull, post-fixed in the same fixative for 45 minutes at 4°C and cryoprotected in sucrose (30% sucrose in phosphate buffered saline (PBS)) overnight at 4°C.
Prior to processing, each brain was released from the skull, post-fixed 1 3 days, and then sent to Neuroscience Associates NSAA, Knoxville, TN) for gelatin embedding and frozen sectioning as described [33].
For the brain slice preparation: the whole brain was removed from the skull, post-fixed in 4% PFA over night, rinsed in PBS (room temperature, 30 min×2), sliced at 50 or 100 µm thickness with a vibrotome (Leica VT1000S), and mounted on slides with the Vectashield mounting medium.
Brains were carefully removed from the skull, post-fixed for 2 h and dissected.
The brains were removed from the skull, post-fixed for 1 h at room temperature (RT), rinsed in PB, cryoprotected in 20 % sucrose overnight, frozen on dry ice and sectioned coronally at 40 µm with a cryostat.
Each brain was removed from the skull, post-fixed in the same fixative for 2 h, and then transferred to 30% sucrose in PB at 4 °C until it sank.
Periosteal stem cells (green) in the mouse skull at post-natal day 15.
Their physical differences, including the architecture of the skull and post-cranial skeletal proportions, do not explain the extinction of the Neanderthals as a species, however.
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