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Exact(11)
A slanted skull position was used to avoid penetrating the lateral ventricles, with the incisor bar set at 5.0 mm above interaural zero.
When a flat skull position was used, the stereotaxic coordinates were 0.9 mm caudal to the bregma, 1.5 mm lateral to the midline, and 3.5 mm (pmch+/+ rats) or 3.0 mm (pmch−/− rats) from the surface of the skull.
An X-ray of the animals in their final position was taken and developed in double exposure (with and without lead shielding) to check the appropriate skull position against an X-ray of the "ideal" position previously confirmed by dose calibration tests.
Details of the stereotaxic device, the procedures to determine the skull position, and the reconstruction of the stimulation and iontophoresis sites are described elsewhere [ 39].
Animals were anaesthetized and secured in a stereotaxic frame (David Kopf Instruments, Tujunga, CA, USA), fitted with atraumatic ear bars, with the incisor bar set at −3.3 mm relative to the interaural line for a flat skull position.
Animals were anaesthetized and secured in a stereotaxic frame (David Kopf Instruments, Tujunga, CA, USA), fitted with atraumatic earbars, with the incisor bar set at −3.3 mm relative to the interaural line for a flat skull position.
Similar(48)
Specimens were photographed with an Olympus SP 350 digital camera with the skull positioned according to the Frankfurt plane.
Anaesthesia by severe hypothermia induces almost no lethality, the injection implicates only a small incision of the skin and skull, positioning of the injection needle is controlled by a stereotaxtic device and the volume of the injected liquid is well defined via a microsyringe.
All stereotactic measurements were made relative to bregma in the flat-skull position.
The coil was held tangentially to the skull and positioned at 45° in relation to the nasion-inion line.
The procedure should ensure an incision less than 5 cm, fixation of the device to the skull, and positioning of the receiver coil such that the auditory stimulator can be worn comfortably behind the ear.
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