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This early change in alternative splicing was not seen for LDLR exon 4 or 12 skipping, or for MVK exon 4 skipping (data not shown).
In contrast to the influence "skipping" data had in the D14_ALP models, the performance of D21_Ca models was enhanced by reducing the frequency of morphological features to 48-h intervals.
First, patients who were excluded from analyses (due to loss to follow-up or skipping data collection) were comparable with persons included in the analyses with respect to baseline characteristics (see Additional file 1: Table S1).
Sequencing of the PCR products confirmed that the smaller band lacked exon 43 sequence, indicating that the G>A mutation is associated with exon 43 skipping (data not shown).
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Twenty-two percent were lost to follow-up and 6%% of the patients skipped data collection at T1 (see Fig. 1).
Baseline characteristics of persons available for analyses versus those lost to follow-up or those who skipped data collection at T1 (N = 749).
Patients who skipped data collection were still willing to participate in future follow-up visits (e.g. at three years follow-up).
The percentage of older cancer and persons without cancer that were lost to follow-up or skipped data collection at one-year follow-up was comparable (p = 0.93).
Of the 503 participants, 116 (23%) were lost to follow-up and 29 (6%) patients skipped data collection at one-year follow-up (see Figure 1).
The proportion of OCP and YCP that were lost to follow-up or skipped data collection at T1 was comparable (p = 0.79).
The LNK exons are as conserved as constitutive exons and the conservation level of the ME exon is roughly equal to the individual cassette exons in exon skipping events with only one exon skipped (data not shown).
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