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Type I collagen derived from tilapia (Oreochromis niloticus) scale and porcine skin were generated from Taki chemical, Japan, and Nitta gelatin, Japan, respectively.
Two variants of embryo-aggregated chimeric mice with either variable or no contribution of C57-derived cells to their skin were generated by the fusion of different ratios of morula stage blastomers.
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Reconstructed skin was generated as described before [36].
Aldehydes in the skin are generated by the oxidation of unsaturated fatty acids released from membrane phospholipids by UV generated ROS.
An additional wavelet model of the skin was generated from the outer surface of the skull, which was obtained using the MNE software extension to the Massachusetts General Hospital's FreeSurfer program.
A total of 48 full-thickness skin defects were generated on six minipigs and treated with the novel collagen gelatin fleece of different thicknesses in single or multiple application (n = 36) or treated with Matriderm® in a single application (n = 6), or the wounds were left untreated (n = 6).
Skin reconstructs were generated using MelanoDerm (MatTek Corp., Ashland, MA, USA) with modifications.
In vitro organotypic skin models were generated as previously described (20).
In vitro skin equivalents were generated as described previously with minor modification (Mildner et al., 2010).
Skin models were generated using 24-well inserts (Nunclon TM Δ Nunc, Rochester, NY, USA) in 24-well plates (Greiner-bio-one).
Skin equivalent cultures were generated as previously described [17].
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