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Bourdet (2002) presented a major development in the mid-1980 in which he advocated superimposing of the log-time derivative of the storage and skin solution on the accepted form of the storage type curve.
Whole-mount airways were fixed with 4% vol/vol paraformaldehyde, washed in 0.1 Triton X-100 in PBS, subjected to antigen retrieval by boiling in 10 mM citrate buffer for 40 min, and incubated in blocking buffer (3 hr; 10% FBS, 0.05% fish skin solution in PBS).
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This variety leads to the issue of designing a software framework able to: (i) provide a unified interface to access information originating from heterogeneous robot skin systems; (ii) assure portability among different robot skin solutions.
The arrays were then transferred to the skinning solution and stored at −20°C.
The bundle was then stored in skinning solution at −20 °C for up to 4 weeks.
After skinning, the fibers were stored on ice in skinning solution without Triton X-100 over night.
For skinning, the fibers were fixed isometrically and incubated in skinning solution on ice for 4 h.
Exposure for 30 min to skinning solution containing Triton X-100 1% was used to ensure membrane solubilization.
They were then treated with skinning solution (relaxing solution containing glycerol; 50 50 v/v) for 24 hours at 4°C, after which they were transferred to −20°C.
Briefly, after animal sacrifice, muscle bundles to be used for single fibre analysis were stored at −20°C in a solution containing skinning solution and glycerol (50%).
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