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The results (Supplementary Table S2) show that the increased functional constraint on amino acid composition does indeed lead to a decreased difference in GC skew between the two species groups.
The nucleotide skew between the two mitochondrial strands is expected to be a combination of various factors, and one could imagine a model whereby a reversal between N. macromphalus and K. tunicata in which strand is leading during replication could account for their differing skew patterns.
The underlying mechanisms resulting in the variation of male reproductive skew between the two species remain unclear at this time, but may result from female choice of male sperm or male-male competition occurring among sperm within the female reproductive tract.
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To compare patterns of W→S DAF skew between the three populations, we calculated pairwise correlations of U-norm in 40 kb and 1 Mb windows across the genome.
The distribution of tumor stages was somewhat skewed between the two groups.
In fact, strand bias has been demonstrated with mutational skews between the two strands, which causes deviation from parity [ 13, 15].
Furthermore, the missingness is highly skewed between the two classes, being significantly higher in the negative class data this limits the choice of imputation techniques that can be used.
It has been shown both in human and in S. cerevisiae that these asymmetries have resulted, after long evolutionary time, in compositional skews between the two DNA strands (i.e., G ≠ C and T ≠ A on one strand) (Chen et al. 2011; Agier and Fischer 2012; Marsolier-Kergoat 2012; Marsolier-Kergoat and Goldar 2012).
Through comparison of nucleotide skew in all sequenced Orthoptera, the skew divergence between the two suborders was detected.
Within each class, the difference in the skewing probability between the two alleles was also much weaker for H3K27me3 as compared to the other proteins.
Thus, the overall correlation in XCI skewing between these two tissues cannot be explained by sample cross-contamination.
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