Sentence examples for skeleton vector from inspiring English sources

The recombinant plasmid was obtained by recombination between the plasmid pShuttle-CMV-MOMP and skeleton vector pAdEasy-1 in Escherichia coli strain BJ5183.

All sense orientation PCR fragments were digested with RsrII plus BamHI and cloned into the same sites of the skeleton silencing vector.

The model is especially designed to produce a vector skeleton of a pattern.

The monocistronic plasmids CMV-LucR and CMV-LucF+ had the same vector skeleton as the bicistronic vectors.

Furthermore, the primers' annealing regions which are complementary to the template are predesigned and unchanged when using the same vector skeleton so that the problem rarely happens.

The problem could be avoided by performing a homology search: the loop and target sequences should not be homologous with the vector skeleton.

A skeleton silencing DNA vector, named 35S:irint, into which a targeted gene could be easily cloned in opposing orientations separated by an intron was made by removing the GUS coding region from pFF19G [ 39] by digestion with Nru1 and Pst1 and replacing the GUS fragment with another containing the castor bean catalase intron 1 [ 40].

For knock-in CRISPR/Cas9, two donor plasmids were constructed based on the skeleton of T-easy vector.

Furthermore, the skeleton of the intermediate vector, containing an ampicillin-resistance gene for bacterial selection and the ColE1 origin of replication, did not need to be removed, and can be used for plasmid rescue procedures after transformation.

We chose the pMD18-T simple vector (Takara) as a skeleton with ampicillin resistance, and MCS from pBluescript SKII.

Finally, the linear vector fragment excluding the skeleton of the donor plasmid was transformed into the SW106 cells to achieve recombineering, and then screened for the correct clone on the right medium.