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By Alcian blue and Alizarin red staining of the skeletons, a mild reduction in the size of the skeleton (data not shown) was noted, but there were no patterning defects observed in the P1 transgenic mice.
However, we were not able to achieve comparable results with bacterial biofilms from Pseudoalteromonas strains A3 and J010 grown on sterile chips of Porites sp. skeleton (data not shown).
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Likewise, the digestive organs and skeleton did not exhibit substantive anatomic abnormalities (data not shown).
These effects on bone tissue were observed at several sites in the skeleton (long bones and vertebrae) (Supplementary Table 1 and data not shown).
Vehicle-treated zebrafish hepatocytes demonstrated basal 6β-, 16α-, 16β-hydroxylationroxylactivitytivity as demonstrated by high-performance liquid chromatography (HPLC) analysis (data not shown; see Figure 1 for numbering of carbon skeleton of testosterone).
Regions of apparently bare skeleton following coral tissue death had oxygen levels typically near or just below ambient (data not shown), while areas of disrupted coral tissue were hypoxic (Figure 5).
Also, torsion misalignment of the axial skeleton was observed in the caudal region, which was not accompanied with obvious inflammation (data not shown).
Assessing the ossified bone volume of several different bones in the skeleton, we found that the double mutants exhibited a decrease in bone volume (Tables 3 and 4) and length (data not shown) of the humerus and femur.
A linkage map skeleton was constructed using 70 SSR markers that were polymorphic between Suweon 542 and Milyang 23 and evenly distributed across the 12 rice chromosomes, with 4 8 markers per chromosome (data not shown).
p.i (data not shown).
(detailed data not shown).
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