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The PCR products were separated in an agarose gel by electrophoresis; amplicons of the appropriate sizes were subsequently excised from the gel and purified using QIAGEN gel extraction kit (Qiagen, Germany).
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These flaw sizes are subsequently used to plot lifetime prediction curves for the bolted connections that are useful for real-world applications.
The changes of aggregated mucin size were subsequently monitored after 3, 24, 48, and 72 hrs.
To examine predictors within the polytherapy subgroup only, unadjusted logistic regression models (due to the small sample size) were subsequently tested.
Two transformed colonies per TDF with an insert of the expected size were subsequently sequenced (Macrogen Inc .. cDNA sequences were manually checked for overall quality, and vector and primer contaminations were trimmed.
The ventral fin is hidden from conspecific observers in this land fish and its size is subsequently not visible for assessment by mates or rivals.
The sample size was subsequently revised to 120 patients following a review of the viability of the HOLLT Study triggered by slow recruitment.
When the sample size was subsequently expanded (n = 58) using samples collected in 2003, no significant correlations were found between B-cell or T-cell proliferations (Table 3).
The pool size was subsequently calculated by multiplying the cumulative amplitude by the mean quantal size of each muscle, confirming a strong reduction following PrP3F4 but also PrPP101L expression (Fig. 5E).
After 40 cycles, the specificity of the amplification was verified by melting curve analysis, and the amplicon size was subsequently confirmed by electrophoresis in 2 % (w/v) agarose gels.
Sphericity, volume and size was subsequently calculated according to the methods proposed by Kelm et al. and Vinci et al. The sphericity of each structure was expressed calculating the Shape Factor:.
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