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Tumor sizes were monitored for 24 days.
The cell mixtures were inoculated into wild-type mice, and tumour sizes were monitored for 43 days.
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Tumor size was monitored for two months after inoculation.
After transplantation of identical cell numbers of both control and TBL1-depleted cells into the flanks of wild-type C57BL/6 mice, distinct animal cohorts were treated with gemcitabine as the most commonly used chemotherapeutic drug in PDAC, and tumor size was monitored for the duration of 3 weeks.
Thereafter tumor sizes were monitored and measured weekly for 6 weeks.
Tumor sizes were monitored every alternate day.
The wound sizes were monitored daily.
Then they were fed ad libitum and their body size and reproductive behavior (egg size, clutch size, reproductive investment) were monitored for two weeks (see Materials and Methods, section B, Experiments 1 and 2, and section C).
In a subsample of 255 cows, ovarian structures were monitored for size and ovulation, and blood samples were collected on study Days 7, 14, 25, 32, 34, and 41 to measure progesterone.
Animals fed with ±Doxycline (Dox) diet (200 mg/kg; Bio-Serv, Flemington, NJ) were monitored for tumor sizes using the Maestro™ In-Vivo Imaging System (CRi, Woburn, MA), as described in the approved Georgetown University IACUC protocol.
Weekly, mice were monitored for tumor size and evidence of morbidity related to the primary tumor or pulmonary metastases.
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