A total of 120,159 contigs (24,781,670 bases in length and 227 bp of contig N50 size) were assembled.
Additionally, 2.74 Mb of mitochondrial sequence, accounting for 95% of the estimated mitochondrial genome size, were assembled into five scaffolds and four additional unscaffolded contigs.
PCR products for fragment sizing were assembled in 96-well microtiter plates at 0.5 ng/μl in each well, with up to six PCR products multiplexed per well according to their predicted allele sizes and fluorescent labels.
End sequences from 600 samples of the cosmid-based library (corresponding to a coverage of more than fivefold of the genome size) were assembled using CAT software, which yielded nine contigs with 97.7% coverage.
In brief, 574 Mb genome size was assembled in 1,174 contigs with an average of 0.49 Mb per contig and 3,256 singletons represent 407 Mb genome.
The genomic content of Krishna Tulsi is 0.72 pg/2C which is equivalent to 704.6 Mb [ 8], but the estimated genome size by k-mer method is 612 Mb and 61%% of the estimated genome size was assembled.
All were callow bees of comparable sizes, and were assembled from several mother colonies to ensure genetic variability.
Given the small number of species data points, therefore, PM data from different size fractions were assembled into a two-dimensional array and analyzed together to significantly increase the number of observations.
The trimmed and size-selected reads were assembled using the Newbler assembly program (Roche).
Approximately 37 million 76-bp paired-end reaverageerage insert size, 400 bp) were assembled into 4411 scaffolds (coverage, ∼94×) using Velvet de novo assembler software.
