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In total, 6 (spheres) × 2 (contrasts) × 4 (reconstruction protocols) × 3 (emission counts) × 10 (replicates) × 2 (matrix sizes) were acquired, making a total of 2,880 objects to be delineated.

Then, three-dimensional (3D) images with 10 × 10  μm sizes were acquired for each group of materials.

To calculate cell numbers, defined sample sizes were acquired and the number of cells normalized as cells/dl of the initial urine sample.

A wide range of infarct sizes were acquired (Fig. 4 B ), and significant correlation was evident between infarct sizes derived from the average of four histological sections sampled from the infarct and four optical OPT slices (r = 0.99, P < 0.0001, n = 8).

Secondly, major sample sizes were acquired by international collaboration (>13 000 individuals in the study of Lambert et al. and >16 000 individuals in the study of Harold et al)., surpassing the power hurdle to detect variants with a minor effect.

Z-stack images with 1-μm step size were acquired at two digital zooms.

For each sample, image stacks (26 images of 0.2-μm Z-step size) were acquired for each wavelength.

Z-stacks with a 0.7- to 1-µm step size were acquired every 8 or 10 min.

Confocal image stacks (Z-stacks) with a 0.63 × 0.63 × 0.65 μm voxel size were acquired for cell volume calculations using Amira 5.4.1 (Visualization Sciences Group; Mérignac, France).

For z-stacks, iso-surfaces were created in surpass mode for both lamin and TO-PRO3; whole nuclei were selected from the 3D image volume and measurements for volume, and object bounding box size were acquired.

After a preliminary evaluation of the AO mirror optical performance, retinal images from 4 normal subjects over a 0.9°x0.9° field size were acquired through a 6.4 mm ocular pupil, showing resolved retinal features at the cellular level.