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The number of genera that would be observed for different sample sizes was estimated using Analytic Rarefaction (version 1.3).
The number of Pfam protein families that would be identified in metagenomes of different sizes was estimated using Analytic Rarefaction.
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Crystal grain sizes were estimated using XRD.
The average crystal sizes were estimated using the Scherrer equation.
Bacterial 16S rRNA gene and fungal ITS population sizes were estimated using qPCR, and bacterial community composition (BCC) was measured using Illumina MiSeq sequencing of 16S rRNA genes.
The extracted DNA sizes were estimated using the DNA marker of GeneRuler™1 kb Plus (Fermentas, Glen Burnie, MD, USA).
Equivalent values of Shannon's diversity index across the same range of sample sizes were estimated using the "H.sampler" function in the DiversitySampler package [63].
Haplotype specific Odds ratios (OR) and effect sizes were estimated using logistic regression for association with EH and linear models with the quantitative phenotypes in R weighted by their posterior probability [30], [31].
Four and a half months later, which approximates the annual length of the reproductive period of voles in Finland, vole population sizes were estimated using Ugglan multiple live capture traps (Grahnab, Sweden).
Effect sizes were estimated using partial η.
Amplicon sizes were estimated using a DNA ladder.
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