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In this case only increased read length, or construction of libraries with larger insert sizes sequenced as mate-pairs, will lead to increased resolution of related genes.
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Simulations of SRS data allow us to validate the method given realistic genome complexity, different genome sizes, sequencing errors, and a range of sequence coverage.
Clone IDs, estimated sizes, sequencing methods, assembly statistics, final contig length, and GenBank accession numbers for resulting contigs are listed in Table 2.
Table 4 Sequencing status of African Oryza genomes Species Genome size Sequencing method Status O. sativa ssp.
In addition, the one-tailed bulk design was less powerful than the two-tailed bulk design at comparable population size, sequencing depth, and heritability.
According to their sizes, sequence repeats may be roughly classified into two main classes.
This taxa-specific bias in the sizes of sequenced genomes partially reflects taxa-specific differences in genome sizes.
However these broad trends in genome size do not fully account for the bias in the sizes of sequenced genomes.
Two samples for each STR locus and two different allelic sizes were sequenced by direct capillary sequencing.
All clones with inserts of expected sizes were sequenced using Macrogen DNA Sequencing service in Korea.
PCR products of expected sizes were sequenced to confirm the deletion.
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