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The percentage of putative SNPs that was validated to be real (SNP validation rate) was found to be directly correlated with contig sizes (number of sequences in the contig) and the minor sequence allele frequencies (Table 4).
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The most important factors for high rate of SNP validation are the contig size (number of sequences in the contig) and the minor sequence allele frequency.
wo factors were found to be most significant for validation of EST-derived SNPs: the contig size (number of sequences in the contig) and the minor allele sequence frequency.
We have found that the contig size (number of sequences in the contig) and minor sequence allele frequency were the two major factors affecting the validation rates of EST-derived SNPs.
Cluster distribution plots are scatter plots where the X-axis is the cluster size (number of sequences in a cluster), and then the Y-axis represents the number of clusters of at least this size and the number of corresponding sequences Figure 1b.
For the assessment of non-randomness, our calculations only require information including genome size, number of (sampled) sequence motifs and distance parameters.
In this case, the analysis was based on the contig size and number of sequences that were placed in assemblies.
It has been observed in Arabidopsis that a number of characteristics such as family size, number of different mature sequences and the length of mature sequence are distinct between conserved and species-specific miRNA families [ 16, 42, 43].
Furthermore, Hox genes depart from a negative correlation found in previous studies between evolutionary rate at the protein level and intron size, number of conserved noncoding sequences within introns, or regulatory complexity [ 32].
We found that the parameters of the mixture distribution are sensitive to 1) the sample size (number of non-redundant sequence tag reads) M, 2) the proportion of nonspecific sequence reads in the sample, 3) the sampling model and 4) the analytical models and the computational algorithm used for the parameterization of the distribution of TF-DNA BEs.
The sensitivity of alignment was not influenced by hash size, and the number of sequences found in the pool rP remained 43 for any hash size used.
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