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Although DLS is a clearer indication of the nanogel sizes in solution, we also show support for the size data using TEM.
Hyperbranched polyglycerol (HPG) and polyethylene glycol (PEG) polymers with similar hydrodynamic sizes in solution were grafted to red blood cells (RBCs) to investigate the impact of polymer architecture on the cell structure and function.
Remarkably, IpaB formed oligomers of similar sizes in solution and bound to LUVs.
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DLS has been used to measure the particle sizes in colloidal solution and the size distribution data of AuNPs obtained at pH 7 from T. conoides and S. tenerrimum are shown in Fig. 4a, b.
Several methods have been employed to control the particle size in solution.
All of these are understood by a compromise effect of ionic affinity to the membrane and its size in solution.
Conjugate size in solution, stability under in vitro and in vivo scenarios and TTR binding affinity through surface plasmon resonance (SPR) was also performed with all synthesised conjugates.
The DLS values listed in Table1 cannot, however, be used to determine the hydrodynamic size in solution in this case as the fluorescent labeling may distort the calculations applied to derive size from light scattering measurements.
DLS results for particle size in solution indicated the chem-AgNPs tended to form agglomerates of greater size than bio-AgNPs when dispersed in either water or cell culture media.
To determine the particle size in solution, dynamic light scattering (DLS) was done for selected materials - graphite (30 nm manufacturer-quoted average particle size (APS)) and silver (20 nm manufacturer APS).
For example, the presence of CaCO3 alters the pH from the optimal value for hydrolysis and Ca2+ ions could bind to the enzymes and neutralize their charges, thus changing their conformation and size in solution.
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