The nucleating effect of CNTs which increased the number of nucleation sites and decreased the average spherulite size was confirmed using polarized optical microscopy.
SMRTbell™ libraries were quantified using the Qubit assay and library size was confirmed using the bioanalyser 12000 kit.
Following amplification, amplicon size was confirmed using the bioanalyser 12000 kit (Agilent, CA, USA) and the concentration confirmed by Qubit assay (Life Technologies, CA, USA).
Libraries were initially quantified by the Quant-IT fluorescent assay (Invitrogen), and fragment size was confirmed using a Bioanalyzer high sensitivity chip in a 2100 Bioanalyzer (Agilent Technologies).
In addition, the amplification of a single product of the expected size was confirmed using 2% agarose gel electrophoresis stained with GelRedTM nucleic acid gel stain (Biotium, California, USA).
The particle sizes were confirmed using a Zetasizer Nano-ZS (Malvern Instruments, Malvern, UK).
Melting temperatures for individual amplicons were determined, and proper PCR product sizes were confirmed using agarose gel electrophoresis.
Amplification was carried out in a DNA thermal cycler (MJ Mini Gradient Thermal Cycler, Bio-Rad Lab, California) and expected fragment sizes were confirmed using gel electrophoresis (2% agarose).
Following recommended protocols, the cDNA was purified and the sizes were confirmed using 2% agarose electrophoresis.
