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This accounted for different "sample size" per individual.
RNAi clones were re-transformed into HT115 DE3) and RNAi was conducted as above, with the exception that single L4 hermaphrodites (n = 12) were placed on individual plates to determine brood size per individual animal.
Two genomic DNA libraries of 500 bp insert size per individual were constructed and sequenced with 100 bp paired-end reads, and each library dataset was generated with five-fold coverage depth.
Histograms showing the distribution of the percentage of rare genome and the number of peaks in the non-outliers and the outliers are shown in Additional file 1: Figure S6.> -wrap-foot> Table shows mean, maximum and minimum number of peaks and total peak size per individual These values can help characterise the two groups of individuals.
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Sample sizes per individual site were small.
For these species, we tested ticks from hosts with >4 newly molted nymphs, but considered these data provisional given low sample sizes per individual host.
To evaluate the power of this approach to detect recent positive selection with the relatively small sample sizes (10 chromosomes per individual population) in this study, simulations were carried out.
We estimated this design effect as (1+ ρ [ θ -1+ θ γ ]), where ρ is the estimated intra-cluster correlation coefficient, θ is the mean sample size per cluster under individual randomization, and γ is the coefficient of variation in the cluster size [ 59].
Mann-Whitney U-tests were used for comparison of CNV size and mean number per individual between cases and controls.
The maximum file size is 5 MB per individual PDF file, and the maximum number of files is 10.
The results of aggregate degradation have been determined by comparing the aggregate gradation in terms of retained aggregate per individual sieve size before and after compaction.
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