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The list of the sequenced BACs and the size of the sequence covered by the pools are shown in the Additional file 4. To simulate the effect of the sequencing depth/coverage on the quality of the assembly, each set of 454 reads from each BAC pool was divided into subsets.
Bands with the length of approximately 1.500 bp correspond to the predicted size of the sequence.
On other hand, at the node j, the number of the frames stored and the total size of the sequence stored are and, respectively.
Before the raw data sequences can be used for automatic landmark point detection and tracking, it is necessary to normalize the size of the sequence such that they were in the format required by the system.
As the Chao1 estimates are dependent on the size of the sequence libraries, the sample sizes from the different subject we equalized by random subtraction.
The optimal size of the sequence tag is a compromise between two opposing goals: while longer sequence tags are more specific, and less likely to occur at multiple positions in the genome, shorter sequence tags make for smaller index files and shorter search times.
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The fragment size of the sequences, annealing temperature (Tm), and real-time PCR efficiency are listed in Table 1.
As memory requirements are dictated mainly by the size of the sequence-context word and read length, they are largely independent of the number of reads processed or simulated.
The mean size of the sequences compared was 1,200 bp.
The more straightforward criterion is the size of the sequences, in which case the longest sequence will be selected.
We show that the size of the sequences that are analyzed can affect the separation of elements into clusters.
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