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Biochemical studies in T. molitor are more abundant because of the larger size of the developmental stages.
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The role of follicle size on the developmental potential of gametes or their fertilization ability has been recognized [ 1, 2, 42, 43].
During size measurements, the developmental stage of embryos in the brood chamber of mature adults, date, and time were recorded to refine estimates of the timing of maturity (Am) and adult instar durations, (A1, A2, A3) [ 45, 58].
While identifying the mechanisms that control spindle size revealed a cellular role in spindle orientation, in the future, it will be interesting to examine how changing spindle size affects the developmental program of the embryo.
In support of this conclusion, studies using human and rodent neurosphere cultures have demonstrated that treatment with ethanol increases neurosphere size, skews the developmental potential of neural progenitor cells, and fundamentally alters the neuronal differentiation program (Roitbak et al. 2011; Vangipuram and Lyman 2012).
In plants, MADS-box genes are of particular interest because of the large size of the family and the critical developmental roles the members are known to play (Theissen et al. 2000).
A composite brightfield and mRFP fluorescence image showed that all of the animals had a detectable red-head that was proportional in area to the developmental stage and size of the animal (Figure 4A).
Although differences in the developmental age and size of the investigated plants have to be taken into account, the present study suggested that stress may increase the AN production or conversion of AN precursors within existing GT, rather than directly influencing the number or size of GT.
It is however not possible to assess this experimentally due to technical limitations associated with the size of the embryonic material at the corresponding developmental stages.
In the meantime, Hatem et al. [ 3] have taken a significant step in resolving the puzzling differences between Manduca and Drosophila, and this work lays the foundation for novel experimental approaches to uncover more fully the details of the developmental physiology of body size regulation.
The size of the miRNAs also differed between the two developmental stages.
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