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For instance, the macroscopic size of the alignment length allows the light diffraction (results not shown).
The synthetic seismograms were realigned in time, which accounts for the deviations of the real velocity structure from the assumed 1D velocity model, so the size of the alignment time correction can be an indication for the validity of the velocity model at distant regions.
Clustering NCIVs also reduces the size of the alignment problem and enables Galinter to obtain results in reasonable run time (within minutes).
The norMD scores also take into account the size of the alignment by calculating the maximum score attainable given the lengths of each of the unaligned sequences and assuming that the sequences are all identical.
Whilst it is not advisable to fit a parameter rich REV model in practice, due to computational constraints and uncertainty in parameter estimates on small alignments, we should aim to derive the best model, given the limitations posed by the size of the alignment.
For phylogenetic studies, extracting polymorphic positions reduced the size of the alignment.
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Specifically, the size of the randomly ordered sequences when compressed by lzip is 826 708 745 bytes while the size of the alignment-ordered raw sequences when compressed by lzip is only 309 46309309
This strict filtering reduced the size of the alignments by approximately 30% (see below).
Accordingly, the size of the alignments used for the GroES-L inter-coevolution analyses ranged between 11 in Cyanobacteria and 215 in Proteobacteria (Table 1).
Due to the large size of the alignments for chromosomes 1 4, the files were split in half; this had no consequence except that an additional step to merge results for each respective chromosome was required.
Given the short size of the V2R alignment (265 aa/sequence), increasing the number of sequences in the alignment will tend to reduce the robustness of branches during phylogeny inference.
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