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In our preliminary investigations, the BSA concentration and volume of BSA solution to total ethanol ratio used for nanocarrier formulation were shown to influence the particle size of formulation.
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The large size of formulations, as well as the loss of flexibility of the therapy, which is essential for controlling metabolic changes and to handle variations in blood pressure, should be also be carefully considered.
The smallest NLC of 155 nm was achieved with the formulation NLC/PI (Fig. 1a, bluebars), whilst the size of the formulation NLC/SI and NLC-GM was significantly larger (compare red and green bars with blue bars).
The size of the formulation did not have significant impact on the bioavailability when both the formulations (SEDDS and SMEDDS) were prepared using medium chain triglycerides.
The particle size of the formulation ESLN-3 was appreciably lower (124.5 ± 3.2 nm) compared to other formulations.
Similarly for atorvaquone the droplet size of the formulation was described as a poor indicator of in vivo performance since various lipid formulations containing different surfactants resulted in different droplet sizes but there was no significant difference in the in vivo results.
The size of optimum formulation was remained almost unchanged (42 nm) after 60 days.
The size of the formulation is further reduced by a preprocessing method that removes suboptimal switches and links from consideration.
The size of the formulation prepared using 40 mg of polymer was lesser and entrapment efficiency was higher of all the three formulations.
Increasing the amount of polymer, beyond an optimum range would result in increase in size of the formulation and an additional amount of energy would be required to reduce the size to desired range.
In this study, a simple modification in the method for the preparation of SLNs helped to increase this ratio to 1 2, and utilizing ultrasonication to produce microemulsions could efficiently compensate the effect of high concentration on stability and size of the formulation.
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