Nevertheless, these approaches ignore invaluable biological pathway information accumulated over decades of research; hence, their selection results can be difficult to interpret biologically and their predictive performance can be limited by a small sample size of expression profiles.
Its only difference to the reduction procedure was to replace the removed genes by three other genes randomly selected from all 5,569 genes, keeping the size of expression profiles unchanged.
We observed that the vast majority of explanted tissue expressed these markers, regardless of when the explant was isolated (Fig. 1E), again with the strength of signal and size of expression domain observed in vivo (Hyatt et al., 1996; Cheng et al., 2000; Essner et al., 2000; Levin et al., 2002).
Expression in the perichondrium and joint capsule remained unchanged and some expression of BMP2 within the patella could be detected although the expression level and size of the expression domain appeared to be reduced (not shown).
To give the researcher the means to control what he really wants to find, features like an unlimited (large) size of search expressions, deep nesting of search subexpressions and truncation operators are necessary.
These segments influence the placement of genes, their length, the probability that a gene is expressed, and the size of the expression level.
The cis-acting expression quantitative trait locus has an effect size of 0.7 log2 expression units per allele, roughly equivalent to a 2.6-fold reduction of Klrd1 mRNA.
For example, a twofold increase in the basal input to Olig2 dramatically expanded the size of its expression domain, whereas a twofold decrease resulted in a dramatic decrease in expression (supplementary material Fig. S6I,J).
In other words, the low overlap between the MA and NI networks can not be explained by the size of the expression sets (n = 5) used in their construction.
During gastrulation the expression of hesx1/anf, six3a, otx2, and zic1/opl were dramatically reduced in intensity and in the size of their expression domains (Fig. 5A H).
