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Gene specific primers were designed using Primer3 (Rozen and Skaletsky 2000) and following criteria: melting temperature 70 °C and product size inferior to 120 bp.
Standard linear regression is difficult to apply in this case due to missing data, which disqualifies for 7 reactions too many datapoints, leaving a dataset of size inferior to the number of parameters to estimate.
In most of the subgroups defined according to these histo-clinical prognostic features (age, lymph nodes, size, grade, HR status), addition of docetaxel reduced the risk of relapse, with significantly greater benefit in patients aged 50 years or older, pathological tumor size inferior to 20 mm, four or more positive lymph nodes, and negative HR status (data not shown).
Characterization of the Z. tritici secretome yielded comparable results to the previously reported Z. tritici secretome (Morais Do Amaral et al. 2012), representing 874 secreted proteins including 441 small, secreted proteins (SSPs), i.e., with a size inferior to 300 amino acids (Table 1).
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NTA assays reveal the presence of a EV population with sizes inferior to 0.3 μm, which reveals that not all the EV population by our FCM are detected.
Therefore, the next tests were conducted using pine sawdust with particle size (d) inferior to 500 µm.
It was also shown that the size of inferior frontal gyrus, particularly pars triangularis can predict rapid naming speed in a group of children with predominating double deficit (Eckert et al. 2005).
The sample size was inferior to the 24 patients indicated by the a priori power analysis as the study was stopped when an interim analysis was conducted due to the large number of dropouts and revealed significant differences for withdrawals between groups.
Further, safety considerations, availability, expense, small group sizes and inferior temporal and spatial resolution (compared to fMRI), have limited the impact of their findings.
This was most pronounced for ND conditions, where vesicle sizes are inferior to experimentally determined values.
As a doctor and keen craniometrist tells her (with regard to the inferior size of the female skull and, ergo, intelligence), "too much intellect would spoil and flatten [the female mind] like a rock in a soufflé".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com